BDCK008 Plant Genomic DNA Extraction Mini Kit ; 植物gDNA小量萃取套組

Brief procedure：

※ Principle : mini spin column (silica matrix)

※ Sample size : 1. Wet weight ≦ 100 mg

                            2. Dry weight ≦ 20 mg

※ Operation time : 30 ~ 60 minutes

※ Binding capacity : up to 60 μg total DNA / column

※ Expected yield : 5 ~ 40 μg /prep

※ Column applicability : centrifugation and vaccum

※ Minimum elution volume : 50 μl

Important Notes :

※ Buffers provided in this system contain irritants. Wear gloves and lab coat when handling these buffers.

※ Check PG1 Buffer before use, Warm PG1 Buffer at 60℃ for 5 min if any precipitate formd.

※ Preheat dry baths or water baths to 65℃ before the operation.

※ Add required ethanol (96-100%) to PG3 Buffer, GW and Wash before use.

※ Store RNase A at -20℃ upon recipit of kit. Add sterile ddH₂O to RNase A tube to make a 50 mg/ml stock solution. Vortex and make sure that RNase A has been completely dissolved. Store the stock solution at -20℃ .

※ All centrifuge steps are done at full speed(~ 18,000 x g) in a microcentrifuge.