BDCK005 Blood Genomic DNA Extraction Mini Kit,含Proteinase K

※ Principle : mini spin column (silica matrix)

※ Sample size : 1. Up to 200 μl whole blood, serum, plasma, body fluids.

                            2. Up to 5 x 10⁶ cultured cells.

※ Operation time : 30 minutes

※ Binding capacity : up to 60 μg total DNA / column

※ Expected yield : 4 ~ 8 μg / prep

※ Column applicability : centrifugation and vaccum

※ Minimum elution volume : 50 μl

Important Notes :

※ Buffers provided in this system contain irritants. Wear gloves and lab coat when

handling these buffers.

※ Add 1.1 ml sterile ddH₂O to Proteinase K tube to make a 10 mg/ml stock solution.

Make sure that Proteinase K has been completely dissolved. Stor e the stock

solution at 4 ℃ .

※ Add ethanol (96- 100 %) to GW Buffer and Wash Buffer when first open.

※ Prepare dry baths or water baths before the operation. One to 60℃ for step 2. Lysis.