PD-L1 細胞染色套組

Product Description

The PD-L1 Stain Kit is intended for laboratory use in the qualitative detection of programmed death ligand 1 (PD-L1) protein via immunofluorescence (IF). This protocol utilizes a biotin-conjugated PDL1 aptamer followed by Alexa Fluor™ 488-conjugated Streptavidin for signal visualization.

The PD-L1 aptamer specifically recognizes the PD-L1 protein and is suitable for immunofluorescence staining of cells to assess PD-L1 expression in the cell membrane or cytoplasm.

Key Features and Advantages

Compared to antibodies, this aptamer-based kit offers the following advantages：

• Versatile Staining Options: The kit is compatible with both fixed and live cells, providing

flexibility for various experimental designs.

• Dynamic Live Staining Mechanism: When used for live cell staining, the aptamer can be

processed via cellular metabolism and transported to the cell surface, enabling unique observation

of cellular dynamics.

• High Specificity and Sensitivity: Ensures accurate detection with high affinity.

• Consistency: Minimal batch-to-batch variability and excellent reproducibility.

Preparation And Storage

1. PD-L1 Aptamer

Please refer to the vial label and reconstitute in nuclease-free water at the concentration of 100 µM. For long-term storage (1 year), aliquot and store at -20ºC. Avoid multiple freeze-thaw cycles. Recommended dilution 1:100 - 1:500.

Pre-staining process： Apply PD-L1 Aptamer (100 µM) in Aptamer Dilution Buffer at 1:100 to 1:500. Heat at 95°C for 10 minutes, then immediately place on ice for 10 minutes.

2. Alexa Fluor 488-conjugated Streptavidin (Green cap)

• Ex/Em maxima of ∼ (495/519).

• Protect from light.

• For short-term storage (6 months), store at 4ºC.

• For long-term storage (1 year), aliquot and store at -20ºC. Avoid multiple freeze-thaw cycles.