PD-L1 Cell Stain Kit 細胞染色試劑組

Product Description

The PD-L1 Cell Stain Kit is intended for laboratory use in the qualitative detection of programmed death ligand 1 (PD-L1) protein via immunofluorescence (IF). This protocol utilizes a biotin-conjugated PD-L1 aptamer followed by Alexa Fluor™ 488-conjugated Streptavidin for signal visualization. The PD-L1 aptamer specifically recognizes the PD-L1 protein and is suitable for immunofluorescence staining of cells to assess PD-L1 expression in the cell membrane or cytoplasm.

Key Features and Advantages

Compared to antibodies, this aptamer-based kit offers the following advantages：

• Versatile Staining Options: The kit is compatible with both fixed and live cells, providing flexibility for various experimental designs.

• Dynamic Live Staining Mechanism: When used for live cell staining, the aptamer can be processed via cellular metabolism and transported to the cell surface, enabling unique observation of cellular dynamics.

• High Specificity and Sensitivity: Ensures accurate detection with high affinity.

• Consistency: Minimal batch-to-batch variability and excellent reproducibility

* Storage temperature: -20 ℃.

* Protect from light and avoid freeze-thaw cycles.

Materials Not Included in the Kit

•Equipment and consumables

1. Micropipettes (10 to 1000 μL)

2. Dry bath

3. Plate shaker

4. Staining reagent： Hoechst 34580

5. Fluorescence microscope

•Buffer

1. Nuclease-free water

2. PBS, pH 7.4

3. 4% Paraformaldehyde

4. Blocking Buffer： 1% BSA in PBS

5. Aptamer Dilution Buffer： 0.55 mM MgCl2 in PBS

Preparation And Storage

1. PD-L1 Aptamer

Please refer to the vial label and reconstitute in nuclease-free water at the concentration of 100 µM. For long-term storage (1 year), aliquot and store at -20ºC. Avoid multiple freeze-thaw cycles. Recommended dilution 1:100 - 1:500.

Pre-staining process： Apply PD-L1 Aptamer (100 µM) in Aptamer Dilution Buffer at 1:100 to 1:500. Heat at 95°C for 10 minutes, then immediately place on ice for 10 minutes.

2. Alexa Fluor 488-conjugated Streptavidin (Green cap)

• Ex/Em maxima of ∼ (495/519).

• Protect from light.

• For short-term storage (6 months), store at 4ºC.

• For long-term storage (1 year), aliquot and store at -20ºC. Avoid multiple freeze-thaw cycles.

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