For printing with Lifeink® 200, we highly recommend using the FRESH printing technique and printing at 4°C. Three-dimensional (3D) gels allow for the study of the effects of the mechanical properties of the extracellular matrix (ECM), such as density and rigidity, on cell development, migration, and morphology. Unlike 2D systems, 3D environments allow cell extensions to simultaneously interact with integrins on all cell surfaces, resulting in the activation of specific signaling pathways. Gel stiffness or rigidity also affects cell migration differently in 3D versus 2D environments.
A bioink that resembles life should have high printability, be shear-thinning, have superior cytocompatibility, allow for cellular remodeling, and be biomimetic. Lifeink® 200 fulfills those requirements.
