Brief procedure:

※ Principle : mini spin column (silica matrix)

※ Sample size : 300 μl

※ Operation time : 30 ~ 60 minutes

※ Binding capacity : up to 100 μg total RNA / column

※ Expected yield : 1 μg

※ Column applicability : centrifugation and vaccum

※ Minimum elution volume : 40 μl

Important Notes :

※ Make sure everything is RNase-free when handling RNA.

※ Buffers provided in this system contain irritants. Wear gloves and lab coat when handling these buffers.

※ Caution: ß-mercaptoethanol is hazardous to human health. perform the procedures involving RB Buffer or PRB Buffer in a chemical fume hood. 

※ Add required volume of RNase-free ethanol (96~100%) to Wash Buffer 2 when first use.

※ Dilute RNase-free DNase 1 in reaction buffer (1M NaCl, 10 mM MnCl2, 20 mM Tris-HCl, pH 7.0 at 25ºC) to final conc. = 0.5 U/μl.