BDC050 RIPA Lysis Buffer

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商品介紹

RIPA Lysis Buffer

產品描述:
RIPA 緩衝液可實現有效的細胞裂解和蛋白質溶解,

同時避免蛋白質降解並幹擾蛋白質的免疫反應性和生物活性。

RIPA緩衝液可使 immunoprecipitation (IP) 和 molecular pull-down assays 中背景值較低。


產品成分:
Ready-to-use solution containing 150 mM NaCl, 1.0% NP-40, 0.5% sodium deoxycholate, 0.1%

SDS, 50 mM Tris, pH 7.5, 1mM PMSF, 10g/ml Leupeptin.


用法:
使用前RIPA Lysis Buffer加入Protease inhibitor cocktail後放置冰上

5 × 106 個細胞,使用 1 mL 冰的 RIPA 緩衝液去收集細胞

(大約20 L of packed cells, 相當於有約40 mg的細胞)

 

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