※ Principle : mini spin column (silica matrix)※ Sample size : 1. Up to 25 mg animal tissue
2. 1.2 cm mouse tail
3. Up to 107 cultured cells
※ Operation time : 30 ~ 60 minutes
※ Binding capacity : up to 60 µg total DNA / column
※ Expected yield : 15 ~35 μg / prep
※ Column applicability : centrifugation and vaccum
※ Minimum elution volume : 50 μl
Important Notes :
※ Buffers provided in this system contain irritants. Wear gloves and lab coat when handling these buffers.
※ Add 1.1 ml sterile ddH2O to Proteinase K tube to make a 10 mg/ml stock solution. Make sure that Proteinase K has been completely dissolved. Store the stock solution at 4 ℃ .
※ Add ethanol (96- 100 %) to GW Buffer and Wash Buffer when first open.
※ Prepare dry baths or water baths before the operation. One to 60℃ for step 2.1 and the other to 70℃ for step 2.2.
※ Preheat the Elution Buffer to 70℃ for step 7.
※ All centrifuge steps are done at full speed(~ 18,000 x g) in a microcentrifuge.