BDCK007-200 Tissue Genomic DNA Extraction Mini Kit,含Proteinase K

※ Principle : mini spin column (silica matrix)

※ Sample size :   1. Up to 25 mg animal tissue

                             2. 1.2 cm mouse tail

                             3.  Up to 10⁷ cultured cells

※ Operation time : 30 ~ 60 minutes

※ Binding capacity : up to 60 µg total DNA / column

※ Expected yield : 15 ~35 μg / prep

※ Column applicability : centrifugation and vaccum

※ Minimum elution volume : 50 μl

Important Notes :

※ Buffers provided in this system contain irritants. Wear gloves and lab coat when handling these buffers.

※ Add 1.1 ml sterile ddH2O to Proteinase K tube to make a 10 mg/ml stock solution. Make sure that Proteinase K has been completely dissolved. Store the stock solution at 4 ℃ .

※ Add ethanol (96- 100 %) to GW Buffer and Wash Buffer when first open.

※ Prepare dry baths or water baths before the operation. One to 60℃ for step 2.1 and the other to 70℃ for step 2.2.

※ Preheat the Elution Buffer to 70℃ for step 7.

※ All centrifuge steps are done at full speed(~ 18,000 x g) in a microcentrifuge.